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KMID : 0377619610010010063
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Korean Jungang Medical Journal
1961 Volume.1 No. 1 p.63 ~ p.85
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The histochemical Study of ascorbic acid in the adrenal cortex following infusion of salt solutions and ACTH which induced the acceleration of blood coagulation and the increase of serum lipids
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Abstract
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Many workers in our laboratory have been studied the accelerating mechanism of blood clotting in vivo by various hemostatics for over 10 years, and they postulated that the acceleration was due to the increase of serum lipids which were liberated from the adrenals by infusion of hemostatics, and the liberation of lipids was completely performed without relation of hypophysis. On the other hand, it is well known fact that ACTH acts in vivo as strong hemostatics and induces marked depletion of fat in the adrenals. The effects of both above mentions hemostatics and ACTH on the adrenal cortex and the blood coagulation were in common, whereas the effect of ACTH to the adrenals is believed to be carried out by physiologic process.
However, the acting mode of hemostatics to the adrenals was obscure. Lo in our laboratory intended to elucidate the acting mode of salts to the adrenals, by means of comparing the behavior of mitochondria in the adrenals of which lipids were markedly deprived by infusions of ACTH and salts. He confirmed that salts caused the depletions of lipids and the degenerative changes in the zona fasciculata of adrenals but could not affect the mitochondria itself, whereas ACTH caused the hypertrophy of zona fasciculata and induced many mitotic cells which contained much larger distinct mitochondria.
From the attitude of mitochondria he concluded that the acting mode of salts to the adrenals was quite different with the effect of ACTH in the adrenals namely the physiologic process.
Author intented to confirm the Lo´s postulation by the different method that is by histochemical study of ascorbic acid in the adrenals. Material and methods; male albino rabbits weighing about 2 kg were used. 1,0 cc/kg.. b. w. L V. injection of solution of each salts. (Calcium chloride, Sodium Citrate, Sodium oxalate and Calcium lactate) The determinations of blood clotting time and serum total cholesterol were respectively Lee and White and Bloor´s method. Histological studies of adrenal cortex were. performed by H-E stain, 70 % Sudan 1 alcohol method, Schultz test and Giroud et Leblond´s method. As part I of this study, the determination of blood clotting time and serum total cholesterol was performed in the normal rabbits, adrenectomized and hypophysectomized rabbits which were infused with salts solutions.
The results were as follows:
1. The salts induced the prominent acceleration of blood coagulation and the increase of serum lipids in the normal rabbits and hypophysectomized rabbits.
2. In the adrenectomized rabbits the effects of salts were inhibited. As part g of this study the determination of blood clotting time and serum total cholesterol in the normal rabbits and adrenectomized rabbits following infusion of ACTH and cortisone emulsion was performed. The results were as follows
1. ACTH induced the marked acceleration of blood clotting and the increase of serum total cholesterol in the normal rabbits but not in the adrenectomized rabbits.
2. However, cortisone emulsion induced, prominent acceleration of blood clotting in the adrenectomized rabbits.
As Part I of this study, the histological studies were performed in the adrenals from the normal rabbits and hypophysectomized rabbits which were treated with salts solutions and ACTH. The results were as follows
1. The salts and ACTH induced the depletion of lipids and ascorbic acid of the adrenals in the normal rabbits and hypophysectomized rabbits.
2. The repeated infusions of calcium chloride induced more marked depletion of lipids
in the zona fasciculata than single infusion, but less diminishing of ascorbic acid than single infusion.
3. However, the repeated infusions of ACTH induced less depletion of lipids in the zona
fasciculata of the adrenals than single infusion and both the repeated and single infusion of ACTH induced marked diminishing of ascorbic acid in the same zone.
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